biotinylated dolichos biflorus agglutinin dba lectin  (Vector Laboratories)

Journal: bioRxiv

Article Title: Multimodal Profiling of Repair-associated Immune Dynamics in a Mouse Model of Menstruation

doi: 10.64898/2026.02.06.704418

Figure Lengend Snippet: Immunohistochemistry: detection of (A) CD3+ T cells, (B) DBA-lectin+ NK cells and (C) B220+ B cells in uterine tissue sections at 0hr (prior to breakdown, n=4), 12hr (tissue breakdown, n=4-6), 24hr (tissue repair, n=4-5) and 48hr (tissue remodelling, n=4-6) hr after progesterone withdrawal (representative images, scale bar=500µm).

Article Snippet: Primary antibodies against CD3 (1:250 dilution; NB600-1441, Novus Biologicals), B220 (1:250 dilution; 14-0452-82, Invitrogen), CD14 (1:6000 dilution; ab221678, Abcam), CD64 (1:6000 dilution; 50086-R008, Sino Biological), Ly6G (1:10000 dilution; 127649, Biolegend) and DBA-lectin (1:1000 dilution; B-1035, Vector) were used in this study.

Techniques: Immunohistochemistry

Journal: Development (Cambridge, England)

Article Title: Defects in nephrogenesis result in an expansion of the Foxd1 + stromal progenitor population

doi: 10.1242/dev.204964

Figure Lengend Snippet: Mutant mouse models with defects in nephrogenesis show an expansion of stroma in the nephrogenic zone. (A-D) Compared to control kidneys (A), mutant mouse models with defects in nephrogenesis including Six2cre;Wt1 c/c , Wnt4-null and Six2cre;RosaDTA c/+ (B-D, respectively) result in decreased kidney size at E15.5. (E-H) Histology confirms fewer nephron structures in mutant models. (I-U) Immunofluorescence (IF) of E15.5 control and mutant kidneys show NPCs labeled with SIX2 (red), early differentiating nephron structures labeled with NCAM (green, M-P; arrows), ureteric bud/collecting ducts labeled with Dolichos biflorus agglutinin (DBA; purple), as well as stroma labeled with MEIS1/2/3 as a global stromal marker (green, I-L) and ALDH1A2 specifically expressed in the stroma surrounding the cap mesenchyme, or ‘nephrogenic zone’ (green, Q-U). Mutant kidneys with defects in nephrogenesis show expansion of stromal cells at the outer periphery of the kidney (arrowheads), with abnormal expression of NCAM (M-P) and expression ALDH1A2, a marker of the nephrogenic zone stroma (Q-T). ALDH1A2 expression at E13.5 (U) does not show similar expansion to mutant kidneys. (V) Quantification of the nephrogenic zone stroma on sections of control and mutant kidneys stained with ALDH1A2 and SIX2 (i.e. as shown in panels Q-T) shows a statistically significant difference between E13.5 and E15.5 control kidneys (** P =0.03), as well as control and mutant kidneys (* P <0.001), with no statistically significant differences noted among the three mutant models. Box plots show median line and first to third interquartile ranges; whiskers indicate 1.5× the interquartile ranges. Representative images are shown from n =3 embryos. Scale bars: 100 μm (E-H); 50 μm (I-U).

Article Snippet: The following primary antibodies were used: SIX2 (Proteintech; 11562-1-AP; 1:200; rabbit; RRID: AB_2189084), SIX2 (Abnova; H00010736-M01; 1:200; mouse; AB_436993), LXH1 (Developmental Studies Hybridoma Bank; 4F2; 1:100; mouse; RRID: AB_531784), NCAM (Sigma-Aldrich; C9672; 1:200; mouse; AB_1079450; recognizes both NCAM1 and NCAM2), CK (DSHB; Troma-III; 1:50; rat; AB_2133570), MEIS 1/2/3 (Active Motif; 39795; 1:100; mouse; RRID: AB_2143020); ALDH1A2 (Sigma-Aldrich; HPA010022; 1:200; rabbit; RRID: AB_1844723), DBA (Vector; B-1035; 1:500; Biotinylated; RRID: AB_2314288), GFP (Aves; GFP-1020; 1:200; chicken; RRID: AB_10000240), PCNA (Abcam; ab18197; 1:200; rabbit; RRID: AB_444313) and pHH3 (Sigma-Aldrich; H0412, 1:500; rabbit; RRID: AB_477043).

Techniques: Mutagenesis, Control, Immunofluorescence, Labeling, Marker, Expressing, Staining